The purpose of CryoEM and CryoET 101 is to teach the principles of both techniques using a media-rich approach with videos, animations, interactive simulations, and real data that cover relevant steps along a typical project workflow. If you are working with purified proteins or protein complexes, we invite you to start with CryoEM 101. If you are interested in imaging cells, subcellular components, or otherwise heterogeneous biological materials, CryoET 101 may be more suitable for you.

CryoEM 101

Ch. 1: Sample Purification

Ch. 2: Cryo-EM Grid Preparation

Ch. 3: Grid Screening & Evaluation

Ch. 4: Cryo-EM Data Collection

Ch. 5: Image Processing

CryoET 101

Ch. 1: Is Cryo-ET for You?

Ch. 2: From Sample to Cryo-ET Grid

Ch. 3: Zoning into Regions of Interest

Ch. 4: Data Collection

Ch. 5: Image Processing & 3D Reconstruction

Ch. 6: Tomogram Annotation & Subtomogram