After your grids are prepared, you are now ready for cryo-EM imaging. All cryo-EM grids need to be screened in order to decide whether resources should be committed towards data collection. The ideal grid contains a rich distribution of monodisperse particles that is supported by a thin layer of vitreous ice. In practice, a number of common issues at the sample or grid level can prevent the justification of data collection. This chapter covers best practices in the screening process, starting from a procedural overview of how grids are loaded into the TEM to assessing whether a grid is ready for data acquisition.
Cryo-EM 101 was created by Peter Shen and Janet Iwasa at the University of Utah. Click here to learn more about the course creators and what they have in store over the coming months.
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